Nucleic Acid Controls
Biolegend Purified anti-E coli RNA Sigma 70 Recombinant [2G10 RecM1] Isotype Mouse IgG1 Reactivity E coli Apps WB Size 100 g
Purified anti-E coli RNA Sigma 70 Recombinant [2G10 RecM1] Isotype Mouse IgG1 Reactivity E coli Apps WB Size 100 g
Zymo Research Corporation Methylated & Non-methylated pUC19 DNA Set, 20 ng
The Non-Methylated pUC19 DNA is pUC19 that was isolated from a methylation-negative strain of bacteria (Dam–, Dcm–) and can be used as a negative control for DNA methylation analysis. The Methylated pUC19 DNA is pUC19 that has been isolated from the same strain and has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2(EC 2.1.1.37; Figure 1) and can be used as a positive control for DNA methylation analysis. The primer set herein has been designed to amplify a fragment of the supplied pUC19 DNAs following bisulfite treatment. The methylated cytosines, comprising CG dinucleotides in the Methylated pUC19 DNA remain unconverted following bisulfite treatment, whereas nonmethylated cytosines are converted into uracil and detected as thymine after PCR. The supplied pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.
Zymo Research Corporation Human Brain DNA, 5 µg/20µL
This set is an ideal control for detection and/or quantification methods against 5- methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) as both modified cytosines are present at physiologically relevant levels and loci.
Zymo Research Corporation Human Spleen DNA (5 ug)
This set is an ideal control for detection and/or quantification methods against 5- methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) as both modified cytosines are present at physiologically relevant levels and loci.
Abcam QX-314 bromide (N-Ethyllidocaine bromide) (mM/ml), Na channel blocker
QX-314 bromide (N-Ethyllidocaine bromide) (mM/ml), Na channel blocker
The product is subject to the following: Abcam Restricted Use Statement
Zymo Research Corporation ZymoBIOMICS Spike-in Control I (High Microbial Load) (500 µl x 10)
When spiked into an unknown sample, this product will serve as an in situ positive control for DNAsequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.
Zymo Research Corporation ZymoBIOMICS Spike-in Control II (Low Microbial Load) (500 µl x 10)
When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Imtechella halotolerans is Gramnegative and Allobacillus halotolerans is Gram-positive while, Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. These species represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.
Cell Signaling Technology SignalSilence Control siRNA (Fluorescein Conjugate) 60 µl
SignalSilence Control siRNA (Fluorescein Conjugate) 60 µl
Biochain Institute Inc Total RNA - Mouse Normal Tissue: Skeletal Muscle, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Fluidigm Corp NUCLEIC ACID INTERCALATOR103RH
Cell-ID Intercalator-103Rh is a cationic nucleic acid intercalator that contains natural-abundance rhodium ( 103Rh) and is used in CyTOF analysis for discrimination of dead cells from live cells (if staining is done before cell fixation) or to discriminate single nucleated cells from doublets (if staining is done post-fixation). It is a live cell membrane-impermeable dye. For CyTOF 2 and Helios instruments we recommend using Cell-ID Intercalator-Ir (201192B) for nucleated cell discrimination and Cisplatin-194 (201194) or -198 (201198) for dead cell discrimination.
Seracare Life Sciences Inc Seraseq ctDNA Complete™ Reference Material AF5%, 5mL
This specific ctDNA Complete product has an allele frequency of 5%. 25 unique multiplexed variants in 16 genes, covering 12 SNVs, 7 INDELs, 3 CNVs, and 3 SVs, in purified and plasma-like matrix formats. 6 different allele frequencies (AF) – WT, 0.1%, 0.5%, 1%, 2.5% and 5%. Allows end-to-end evaluation of assay performance across the entire workflow, including pre-analytic extraction steps. Variants quantitated with digital PCR and orthogonally validated by NGS. Blends with well-characterized GM24385 human genomic DNA as background ‘wild-type’ material. Manufactured within cGMP compliant, ISO 9001 and ISO 13485 certified facilities.